I. Isolation and Crystallization of the Enzyme?

Greenberg and associates (2, 3) in pigeon liver homogenates and was later found in a variety of bacterial species, including Clostridium cylindrosporum (4), Micrococcus aerogenes (5), and others (6). The enzyme has been purified from acetone powders of pigeon liver (7), from Micrococcus aerogenes (6), and from Clostridium cylindrosporum (8, 9). This paper describes the purification of the enzyme from Clostridium acidi-urici and Clostridium cylindrosporum and the isolation of the enzyme in crystalline form from the latter organism. The properties of the enzyme, the characteristics of the enzymic reaction, and studies on the mechanism of the reaction are described in succeeding papers (1, 10).